Coding

Part:BBa_K558008:Design

Designed by: Megan Tabor   Group: iGEM11_Nevada   (2011-09-22)

Bay Laurel Thioesterase coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 302
    Illegal BsaI.rc site found at 712


Design Notes

Codon-optimizing was done on the Bay Laurel Thioesterase gene to increase the probability of the E.coli to produce a protein from a plant gene. Codons were also changed to rid the sequence of illegal restriction sites. PCR was used to isolate the coding region from the intermedite part BBa K558003.

Source

Umbellularia californica

References

T.A. Voelker, T.R. Hayes, A.C. Cranmer, H.M. Davies [1996] Plant J 9: 229–241 Welch et. al. “Design Parameters to Control Synthetic Gene Expression in Escherichia coli”